Sie sind hier: Startseite AG Prof. Dr. G. Layer Publikationen


der Arbeitsgruppe von Prof. Dr. Gunhild Layer


2020  2019   2018   2017   2016   2014   2013   2012   2011   2010   2009   vor 2009



Layer G. (2020) Heme biosynthesis in prokaryotes.

BBA - Mol. Cell Res., in press.

Layer G. (2020) Radical S-adenosylmethionine enzymes in heme biosynthesis.

In: Hung-Wen (Ben) Liu and Tadhg P. Begley (eds.), Comprehensive Natural Products III: Chemistry and Biology, Volume 5, 349-363.

Jasper J., Vazquez Ramos J., Trncik C., Jahn D., Einsle O., Layer G. & Moser J. (2020) Chimeric interaction of nitrogenase-like reductases with the MoFe protein of nitrogenase.

ChemBioChem, 21, 1733-1741.

Klünemann T., Nimtz M., Jänsch L., Layer G. & Blankenfeldt W. (2020) Crystal structure of NirF: insights into its role in heme d1 biosynthesis.

FEBS J., in press.

Deobald D., Hanna R., Shahryari S., Layer G. & Adrian L. (2020) Identification and characterization of a bacterial core methionine synthase.

Sci. Rep., 10, 2100.


Fan T., Grimm B. & Layer G. (2019) Porphyrin and heme synthesis.

in "Advances in Botanical Research", 91, 89-131.

Klünemann T., Preuß A., Adamczack J., Rosa L.F.M., Harnisch F., Layer G. & Blankenfeldt W. (2019) Crystal structure of dihydro-heme d1 dehydrogenase NirN from Pseudomonas aeruginosa reveals amino acid residues essential for catalysis.

J. Mol. Biol., 431, 3246-3260.

Moser J. & Layer G. (2019) Enzymatic systems with homology to nitrogenase: Biosynthesis of bacteriochlorophyll and coenzyme F430.

Methods Mol. Biol., 1876, 25-35.

Moser J., Jasper J., Vazquez Ramos J., Sowa S.T. & Layer G. (2019) Expression, purification, and activity analysis of chlorophyllide oxidoreductase and Ni2+-sirohydrochlorin a,c-diamide reductase.

Methods Mol. Biol., 1876, 125-140.


Deobald D., Adrian L., Schöne C., Rother M. & Layer G. (2018) Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase.

Sci. Rep., 8, 7404. 


Boss L., Oehme R., Billig S., Birkemeyer C. & Layer G. (2017) The Radical SAM enzyme NirJ catalyzes the removal of two propionate side chains during heme d1 biosynthesis.

FEBS J., 284, 4314-4327.

Moore S.J., Sowa S.T., Schuchardt C., Deery E., Lawrence A.D., Ramos J.V., Billig S., Birkemeyer C., Chivers P.T., Howard M.J., Rigby S.E., Layer G. & Warren M.J. (2017) Elucidation of the biosynthesis of the methane catalyst coenzyme F430.

Nature, 543, 78-82.

Layer G., Krausze J. & Moser J. (2017) Reduction of chemically stable multibonds: Nitrogenase-like biosynthesis of tetrapyrroles.

Adv. Exp. Med. Biol., 925, 147-161.


Layer G. (2016) Siroheme decarboxylase in complex with iron-uroporphyrin III.

in "Encyclopedia of Inorganic and Bioinorganic Chemistry", ed R.A. Scott, John Wiley, Chichester.

Kühner M., Schweyen P., Hoffmann M., Vazquez Ramos J., Reijerse E.J., Lubitz W., Bröring M. & Layer G. (2016) The auxiliary [4Fe-4S] cluster of the Radical SAM heme synthase from Methanosarcina barkeri is involved in electron transfer.

Chem. Sci., 7, 4633-43.


Adamczack J., Hoffmann M., Papke U., Haufschildt K., Nicke T., Bröring M., Sezer M., Weimar R., Kuhlmann U., Hildebrandt P. & Layer G. (2014) NirN from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of heme d1 biosynthesis.

J. Biol. Chem., 289, 30753-62.

Haufschildt K., Schmelz S., Kriegler T.M., Neumann A., Streif J., Arai H., Heinz D.W. & Layer G. (2014) The crystal structure of siroheme decarboxylase in complex with iron-uroporphyrin III reveals two essential histidine residues.

J. Mol. Biol., 426, 3272-86.

Kühner M., Haufschildt K., Neumann A., Storbeck S., Streif J. & Layer G. (2014) The alternative route to heme in the methanogenic archaeon Methanosarcina barkeri.

Archaea, 2014, Article ID 327637.


Nicke T., Schnitzer T., Münch K., Adamczack J., Haufschildt K., Buchmeier S., Kucklick M., Riedel K., Felgenträger U., Jänsch L. & Layer G. (2013) Maturation of the cytochrome cd1 nitrite reductase NirS from Pseudomonas aeruginosa requires transient interactions between the three proteins NirS, NirN and NirF.

Biosci. Rep., 33, art:e00048.

Kaufholz A.-L., Hunter G. A., Ferreira G. A., Lendrihas T., Hering V., Layer G., Jahn M. & Jahn D. (2013) Aminolevulinic acid synthase of Rhodobacter capsulatus - high resolution kinetic investigation of the structural basis for substrate binding and catalysis.

Biochem. J., 451, 205-16.


Abicht H.K., Martinez J., Layer G., Jahn D. & Solioz M. (2012) Lactococcus lactis HemW (HemN) is a heme-binding protein with a putative role in heme trafficking.

Biochem. J., 442, 335-43.


Layer G. & Warren M.J. (2011) Biosynthesis of siroheme, cofactor F430 and heme d1.

Handbook of Porphyrin Science19, 111-38.

Storbeck S., Saha S., Krausze J., Klink B.U., Heinz D.W. & Layer G. (2011) Crystal structure of the heme d1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine dependent uroporphyrinogen III methyltransferases.

J. Biol. Chem., 286, 26754-67.

Layer G., Jahn D. & Jahn M. (2011) Heme biosynthesis.

Handbook of Porphyrin Science15, 159-215.


Storbeck S., Rolfes S., Raux-Deery E., Warren M.J., Jahn D. & Layer G. (2010) A novel pathway for the biosynthesis of heme in Archaea: Genome-based bioinformatic predictions and experimental evidence.

Archaea, 2010, ID 175050.

Wollers S., Layer G., Garcia-Serres R., Signor L., Clemancey M., Latour JM., Fontecave M. & Ollagnier-de Choudens S. (2010) Iron-sulfur (Fe-S) cluster assembly: the SufBCD complex is a new type of Fe-S scaffold with a flavin redox cofactor.

J. Biol. Chem., 285, 23331-41.

Layer G., Reichelt J., Jahn D. & Heinz D.W. (2010) Structure and function of enzymes in heme biosynthesis.

Protein Science, 19, 1137-61.

Rand K., Noll C., Schiebel H.M., Kemken D., Dülcks T., Kalesse M., Heinz D.W. & Layer G. (2010)  Oxygen-independent coproporphyrinogen III oxidase HemN utilizes harderoporphyrinogen as a reaction intermediate during conversion of coproporphyrinogen III into protoporphyrinogen IX.

Biol. Chem., 391, 55-63.

Layer G., Jahn D., Deery E., Lawrence A.D. & Warren M.J. (2010) Biosynthesis of heme and vitamin B12.

in "Comprehensive Natural Products II, Chemistry and Biology" (Mander, L., Lui, H.-W.) Elsevier: Oxford; 7, 445–99.


Storbeck S., Walther J., Müller J., Parmar V., Schiebel H.M., Kemken D., Dülcks T., Warren M.J. & Layer G. (2009) The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis.

FEBS J., 276, 5973-82.

vor 2009

Layer G., Gaddam S.A., Ayala-Castro C.N., Ollagnier-de Choudens S., Lascoux D., Fontecave M. & Outten F.W. (2007) SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly.

J. Biol. Chem., 282, 13342-50.

Layer G., Ollagnier-de Choudens S., Sanakis Y. & Fontecave M. (2006) Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CyaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU.

J. Biol. Chem., 281, 16256-63.

Layer G., Pierik A.J., Trost M., Rigby S.E., Leech H.K., Grage K., Breckau D., Astner I., Jänsch L., Heathcote P., Warren M.J., Heinz D.W. & Jahn D. (2006) The substrate radical of Escherichia coli oxygen-independent coproporphyrinogen III oxidase HemN.

J. Biol. Chem., 281, 15727-34.

Reents H., Gruner I., Harmening U., Böttger L.H., Layer G., Heathcote P., Trautwein A.X., Jahn D. & Härtig E. (2006) Bacillus subtilis Fnr senses oxygen via a [4Fe-4S] cluster coordinated by three cysteine residues without change in the oligomeric state.

Mol. Microbiol., 60, 1432-45.

Layer G., Grage K., Teschner T., Schünemann V., Breckau D., Masoumi A., Jahn M., Heathcote P., Trautwein A.X. & Jahn D. (2005) Radical S-adenosylmethionine enzyme coproporphyrinogen III oxidase HemN: functional features of the [4Fe-4S] cluster and the two bound S-adenosyl-L-methionines.

J. Biol. Chem., 280, 29038-46.

Layer G., Kervio E., Morlock G., Heinz D.W., Jahn D., Retey J. & Schubert W.D. (2005) Structural and functional comparison of HemN to other radical SAM enzymes.

Biol. Chem., 386, 971-80.

Layer G., Heinz D.W., Jahn D. & Schubert W.D. (2004) Structure and function of Radical SAM enzymes.

Curr. Opin. Chem. Biol., 8, 468-76.

Layer G., Moser J., Heinz D.W., Jahn D. & Schubert W.D. (2003) Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes.

EMBO J., 22, 6214-24.

Breckau D., Mahlitz E., Sauerwald A., Layer G. & Jahn D. (2003) Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia coli is stimulated by manganese.

J. Biol. Chem., 278, 46625-31.

Baysse C., Matthijs S., Schobert M., Layer G., Jahn D. & Cornelis P. (2003) Co-ordination of iron acquisition, iron porphyrin chelation and iron-protoporphyrin export via the cytochrome c biogenesis protein CcmC in Pseudomonas fluorescens.

Microbiology, 149, 3543-52.

Layer G., Verfürth K., Mahlitz E. & Jahn D. (2002) Oxygen-independent coproporphyrinogen-III oxidase HemN from Escherichia coli.

J. Biol. Chem., 277, 34136-42.